Journal: Journal of Clinical Medicine

Article Title: Examining the Potential Link Between Forkhead Box P1 and Severity and Social Impairment in Children with Autism Spectrum Disorder

doi: 10.3390/jcm14207132

Figure Lengend Snippet: Median FOXP1 (pg/mL) levels in ASD and control groups.

Article Snippet: The plasma levels of FOXP1 were evaluated using commercially available sandwich ELISA kits (Cusabio Biotech Co. Ltd., Wuhan, China, Cat# E15456h), according to the manufacturer’s instruction.

Techniques: Control

Journal: Journal of Clinical Medicine

Article Title: Examining the Potential Link Between Forkhead Box P1 and Severity and Social Impairment in Children with Autism Spectrum Disorder

doi: 10.3390/jcm14207132

Figure Lengend Snippet: Correlation between FOXP1 protein (pg/mL) and CARS in ASD group.

Article Snippet: The plasma levels of FOXP1 were evaluated using commercially available sandwich ELISA kits (Cusabio Biotech Co. Ltd., Wuhan, China, Cat# E15456h), according to the manufacturer’s instruction.

Techniques:

Journal: Journal of Clinical Medicine

Article Title: Examining the Potential Link Between Forkhead Box P1 and Severity and Social Impairment in Children with Autism Spectrum Disorder

doi: 10.3390/jcm14207132

Figure Lengend Snippet: Correlation between FOXP1 protein (pg/mL) and SRS in ASD group.

Article Snippet: The plasma levels of FOXP1 were evaluated using commercially available sandwich ELISA kits (Cusabio Biotech Co. Ltd., Wuhan, China, Cat# E15456h), according to the manufacturer’s instruction.

Techniques:

Journal: Journal of Clinical Medicine

Article Title: Examining the Potential Link Between Forkhead Box P1 and Severity and Social Impairment in Children with Autism Spectrum Disorder

doi: 10.3390/jcm14207132

Figure Lengend Snippet: Correlation between FOXP1 protein (pg/mL) and age in ASD group.

Article Snippet: The plasma levels of FOXP1 were evaluated using commercially available sandwich ELISA kits (Cusabio Biotech Co. Ltd., Wuhan, China, Cat# E15456h), according to the manufacturer’s instruction.

Techniques:

Journal: Clinical and Translational Medicine

Article Title: T‐cell differentiation stage block bias confers hypermethylation and mediastinal preference in T‐cell lymphoblastic lymphoma

doi: 10.1002/ctm2.70380

Figure Lengend Snippet: UHRF1 affects the malignancy of T‐cell lymphoblastic lymphoma (T‐LBL) through DNA methylation. (A) Scatter plot indicating differentially methylated loci (DMLs) in Uhrf1‐sufficient and Uhrf1‐deficient T cells, with red dots representing the DMLs. (B) Scatter plot displaying DEGs in Uhrf1‐sufficient and Uhrf1‐deficient T cells, with red dots representing the DEGs. (C) Metascape network enrichment analysis of intersected DMLs and DEGs, displaying the top 20 enriched terms; dot colours indicate different enriched terms. (D) Scatter plot indicating differentially methylated regions (DMRs) in vector‐ and UHRF1‐knockdown cells, with red dots representing the DMRs. (E) Scatter plot indicating DEGs in vector‐ and UHRF1‐knockdown cells, with red dots representing the DEGs. (F) Heatmap illustrating the Pearson correlation between target downstream genes and UHRF1 in the scT‐LBLs. (G) Methylation profile of the target genes of UHRF1 in vector control and UHRF1‐knockdown cells, namely, FOXP1 , PIK3R1 and KLF6 . H. Verification of target proteins of UHRF1 by WB analysis in vector‐ and UHRF1‐knockdown cells. FOXP1, PIK3R1 and KLF6 were significantly upregulated following UHRF1 knockdown. The data are presented as the means ± SDs, and p values were calculated by two‐tailed t ‐test.

Article Snippet: For mouse tumour slides, we examined changes in target protein expression following demethylation treatment with the following antibodies: FOXP1 (1:200, A23442, ABclonal), PIK3R1 (1:200, 60225‐1‐Ig, Proteintech) and KLF6 (1:200, A10011, ABclonal).

Techniques: DNA Methylation Assay, Methylation, Plasmid Preparation, Knockdown, Control, Two Tailed Test

Journal: bioRxiv

Article Title: Temporally-segregated dual functions for Gfi1 in the development of retinal direction-selectivity

doi: 10.1101/2025.06.03.657700

Figure Lengend Snippet: Gfi1 is expressed in direction-selective D-oDSGC and F-mini ON RGC subsets ( A ) Schematic depicting Gfi1 expression in the retina restricted to D-oDSGCs, F-mini-ONs and W3Bs. D-oDSGCs and F-mini ONs are direction-selective subsets tuned primarily to downward motion, while W3Bs are non-direction-selective. ( B ) Wholemount P6 Gfi1 Cre , LSL-YFP retina shows YFP expression restricted to Rbpms + RGCs. ( C ) Density recovery profile (DRP) of YFP + RGCs suggests an absence of mosaicism confirming Gfi1 expression in more than one RGC subtype. ( D ) Density of Gfi1-expressing RGCs in the retina. ( E ) In situ hybridization in P4 retina cryosection shows Gfi1 expression in Fibcd1 + D-oDSGCs. ( E’, E” ) Detailed views of the nuclei indicated in D. ( F ) Quantification of the percentage of Gfi1 + RGCs co-expressing Fibcd1 . ( G ) Coronal brain section from Gfi1 Cre , LSL-tdTomato adult mouse shows strong innervation of the ventral medial terminal nucleus (MTN) by tdTomato + RGCs. ( H-N ) Detailed views of all retinorecipient nuclei labeled with cholera toxin B (CTB) from Gfi1 Cre , LSL-YFP adult mice confirms YFP + RGC innervation exclusively in the ventral MTN (H, H’ ) and the SC ( I, I’ ), and not in the other retinorecipient nuclei ( J-N ). ( O ) Wholemount P1 Gfi1 Cre , LSL-YFP retina shows YFP expression in a subset of Foxp2 + RGCs. ( P, Q ) These YFP + Foxp2 + RGCs account for ∼25% of all Foxp2 + RGCs and ∼50% of all Gfi1 + RGCs. ( R ) Combinatorial labeling can differentiate between F-RGC subsets. ( S-U ) Wholemount Gfi1 Cre , LSL-YFP retina shows that YFP + Foxp2 + RGCs do not express the F-RGC OFF marker Foxp1 or the F-midi ON marker Brn3c, confirming their F-mini ON identity. Data are presented as mean ± SE.

Article Snippet: Primary antibodies used in this study include: Rabbit anti-dsRed (Living Colors, 1:1000), Chick anti-GFP (AVES, 1:1000), Guinea Pig anti-Foxp2 (Synaptic Systems, 1:500), Rabbit anti-Rbpms (Proteintech, 1:500), Guinea Pig anti-Rbpms (ThermoFisher, 1:500), Mouse anti-Brn3c (Santa Cruz, 1:100), Rabbit anti-Foxp1 (Proteintech, 1:500), Rabbit anti-IBA1 (Wako Laboratory Chemicals, 1:500), Goat anti-ChAT (Abcam, 1:250), Goat anti-VAChT (Millipore, 1:250) and Rabbit anti-cFos (Cell Signaling Technology, 1:500).

Techniques: Expressing, In Situ Hybridization, Labeling, Marker

Journal: bioRxiv

Article Title: Evidence for chronological diversification of spinal neuron subtypes by a shared sequence of transcription factors

doi: 10.1101/2025.05.27.656404

Figure Lengend Snippet: (A) Cervical and thoracic MNs are partitioned into distinct motor columns that express different marker genes (B) OPP mRNA expression relative to motor column markers Isl1 , Isl2 , Lhx3 , Lhx1 and Foxp1 in MN scRNAseq data from Delile et al. 2019. Expression of additional marker genes is shown in . (C) OPP expression in MNs at e10.5. Consistent with their sequential generation, OPP+ MNs are distributed in stripes along the mediolateral axis of the spinal cord at e10.5. (D) Staining for the MMC marker Lhx3 (red), and the LMC marker Foxp1 (blue) and the temporal TF Pou2f2 (green) in MNs identified by Isl1/2 staining at e10.5. (E) Intensity distribution of OPP TFs and motor column markers (Isl1/2, Lhx3, Foxp1) along the mediolateral axis in motor neurons at e10.5. OC2 correlates with Foxp1 expression and lateral upregulation of Isl1/2 indicative of an LMCm identity. Pou2f2 expression correlates with a stripe of reduced Isl1/2 staining, indicative of the LMCl, while Pou3f1 expression correlates with Lhx3 expression in the most medial population of MNs. (F) Pou2f2 expression precedes expression of the LMCl marker Lhx1 in MNs. (G) Expression of the MMC marker Mecom is limited to few Onecut2+ MNs at e10.5. (H) Summary of OPP expression in cervical MNs at e10.5 and e11.5. OC2 neurons largely contribute to the LMCm and a small subset of MMC MNs, Pou2f2 to the LMCl, and Pou3f1-positive neurons to the MMC. (I) OC2 and Pou2f2 expression partitions the LMC into LMCm and LMCl. OC2 expression in Foxp1+ LMC neurons correlates with expression of the LMCm marker Isl1/2. Pou2f2 expression correlates with down-regulation of Isl1/2 and LMCl identity. (J) OC2 levels are elevated in the LMCm compared to the LMCl. (K) Quantification of the percentage of Pou2f2+ and Pou3f1+ neurons in the LMCm, LMCl, MMC (Isl1/2+ Foxp1-) and Mecom+ MMC neurons. (L - N) Pou2f2 and Pou3f1 expression in distinct motor columns. e11.5 cervical MNs were stained for Pou2f2 and Pou3f1 in combination with motor column markers. (L) Pou2f2 is expressed in Isl1/2- Foxp1+ LMCl neurons. Pou3f1 expression is limited to Isl1/2+ Foxp1-MMC neurons. (M) Co-expression between Pou2f2 and Lhx1 in Isl1/2- Foxp1+ LMCl neurons (N) Co-expression between Pou3f1 and Mecom in MMC neurons. Scale bars = 50 µm

Article Snippet: We did not observe increased numbers of MMC MNs (Mecom+ or Foxp1-/Isl1/2+) normally expressing the next temporal TF in sequence, Pou3f1 ( and ).

Techniques: Marker, Expressing, Staining

Journal: bioRxiv

Article Title: Evidence for chronological diversification of spinal neuron subtypes by a shared sequence of transcription factors

doi: 10.1101/2025.05.27.656404

Figure Lengend Snippet: (A) OPP expression in MNs at e10.5 (single channel views of ). (B) Expression of Pou2f2 relative to Isl1/2, MMC marker Lhx3 and LMC marker Foxp1 (single channel views of ). (C) Downregulation of Isl1/2 in the Pou2f2 stripe precedes high-level Lhx1 expression in MNs (single channel views of ). (D) Early occurrence of a small population of Isl1/2+ Mecom+ OC2+ MMC neurons (single channel views of ). Scale bars = 50 µm

Article Snippet: We did not observe increased numbers of MMC MNs (Mecom+ or Foxp1-/Isl1/2+) normally expressing the next temporal TF in sequence, Pou3f1 ( and ).

Techniques: Expressing, Marker

Journal: bioRxiv

Article Title: Evidence for chronological diversification of spinal neuron subtypes by a shared sequence of transcription factors

doi: 10.1101/2025.05.27.656404

Figure Lengend Snippet: (A) e11.5 section stained for Isl1/2 and goat anti-Foxp1 (AF4534) and rabbit anti-Foxp2 (ab16046) antibodies. The Foxp2 antibody gave a strong signal in MNs, which normally do not express Foxp2 (Dasen et al., 2008), and a comparably much weaker signal in a more dorsal population, presumably V1 neurons (white arrowheads), suggesting the antibody detects Foxp1 and Foxp2. Contrast for Foxp1/2 and Foxp1 in the last panel was enhanced compared to the other panels to improve the visibility of the signal in the more dorsal cell population. (B, C) Pou2f2 is expressed in LMCl neurons (Isl1/2-negative Foxp1-positive) and Pou3f1 in MMC neurons (Foxp1-negative) (B; single channel views of ). Consistent with these observations, Pou2f2 and Lhx1 stainings coincide in Foxp1+ LMC neurons (C; single channel views of ). (D) Pou3f1 is expressed in Isl1, Mecom double-positive MMC neurons (single channel views of ). Many of these Isl1, Mecom, Pou3f1 triple-positive neurons still migrate laterally from the pMN to the MMC (arrowheads). (E, F) e9.5 to e12.5 time-course of LMC and MMC neuron formation. (E) MNs stained for Isl1/2, LMC marker Foxp1 and MMC marker Mecom. (F) Quantification of the percentage of MNs expressing the respective markers. Fraction of Foxp1+ neurons increases between e9.5 and e10.5 and stays afterwards constant, while the fraction of Mecom+ neurons only begins to increase after e10.5 (n e9.5, e10.5 = 23 sections from 6 embryos, n e11.5 = 17 sections from 5 embryos, n e12.5 = 6 sections from 3 embryos). Scale bars in A, B, C, D, E = 50 µm; in E (e9.5) = 25 µm

Article Snippet: We did not observe increased numbers of MMC MNs (Mecom+ or Foxp1-/Isl1/2+) normally expressing the next temporal TF in sequence, Pou3f1 ( and ).

Techniques: Staining, Marker, Expressing

Journal: bioRxiv

Article Title: Evidence for chronological diversification of spinal neuron subtypes by a shared sequence of transcription factors

doi: 10.1101/2025.05.27.656404

Figure Lengend Snippet: (A) e11.5 thoracic MNs stained for OC2, Pou2f2, Pou3f1 and the MN marker Isl1/2. (B) e11.5 thoracic MNs stained for Isl1/2, Lhx3, Pou2f2 and Foxp1. Pou2f2 is co-expressed with Foxp1, suggesting Pou2f2+ neurons form the PGC. (C) Co-expression between the Pou2f2 and the PGC marker nNOS in e12.5 thoracic MNs. (D) Quantification of the percentage of Pou2f2+ and Pou3f1+ neurons in the PGC, HMC and MMC at e13.5 (n = 6 sections from 3 embryos). (E) e13.5 thoracic spinal cord section stained for Pou2f2, Pou3f1, the PGC marker nNOS and the MN marker Isl1. (F) Proposed model for the temporally segregated generation of motor columns at different axial levels. Pou2f2+ MNs form the brachial LMCl and thoracic PGC. Pou3f1 is expressed in MMC neurons at both axial levels. OC2 is expressed and functionally required for the formation of the LMCm (Roy et al. 2012). Thoracic OC2+ neurons may contribute to the HMC. Scale bars in E = 50 µm and 25 µm

Article Snippet: We did not observe increased numbers of MMC MNs (Mecom+ or Foxp1-/Isl1/2+) normally expressing the next temporal TF in sequence, Pou3f1 ( and ).

Techniques: Staining, Marker, Expressing

Journal: bioRxiv

Article Title: Evidence for chronological diversification of spinal neuron subtypes by a shared sequence of transcription factors

doi: 10.1101/2025.05.27.656404

Figure Lengend Snippet: (A, B) Reduced number of LMCl neurons and increased number of LMCm neurons in Pou2f2 mutants at e11.5. (A) e11.5 cervical spinal cord sections of control and Pou2f2 mutant embryos stained for the MMC marker Mecom (red), LMC marker Foxp1 (blue) and LMCl marker Lhx1 (green). (B) Number of cells per hemisection for LMCl (i), MMC (ii), LMCm (iii) and LMC (iv) neurons in control and Pou2f2 mutant embryos in cervical spinal cord sections at e11.5. Loss of Pou2f2 causes a significant reduction of LMCl neurons but does not affect the number of MMC, LMCm and LMC neurons. (C, D) Loss of Pou3f1 affects MMC neuron specification. (C) e11.5 cervical spinal cord section stained for the MMC neuron marker Mecom, LMC marker Foxp1 and MN marker Isl1/2. (D) Loss of Pou3f1 causes a significant reduction on the number of Mecom+ MMC neurons (i) but does not affect the number of Isl1/2+ Foxp1-MMC neurons. (E, F) Loss of Pou2f2 affects the specification of thoracic PGC neurons. (E) e13.5 thoracic spinal cord section stained for DAPI and the PGC marker nNOS (red). (F) Loss of Pou2f2 causes a significant reduction of laterally localized nNOS neurons. In B, D, F, Student’s t-test; *p<0.05, **p < 0.01; ***p < 0.001. All data represented as mean ± SEM. Scale bars in A = 50 μm, C and E = 100 μm.

Article Snippet: We did not observe increased numbers of MMC MNs (Mecom+ or Foxp1-/Isl1/2+) normally expressing the next temporal TF in sequence, Pou3f1 ( and ).

Techniques: Control, Mutagenesis, Staining, Marker